Basic HTML Version
252
Kc
R
θ
=
1
+
16
π
2
R
G
2
3
λ
2
sin
2
θ
2
⎛
⎝⎜
⎞
⎠⎟
+
...
⎛
⎝⎜
⎞
⎠⎟
1
M
w
+
2
A
2
c
+
...
⎛
⎝⎜
⎞
⎠⎟
⇒
Slope of fitted line =
16
π
2
R
G
2
3
λ
2
⎛
⎝⎜
⎞
⎠⎟
1
M
w
+
2
A
2
c
+
...
⎛
⎝⎜
⎞
⎠⎟
R
G,i
is then calculated directly since the other factors are known.
The data matrix is now extended to include M
w,I
and R
G,i
for each elution slice:
Raw data
Calculated
Slice V
(ml)
c
i
(g/ml)
i
1
i
2
… i
18
Kc
i
/R
θ
M
i
R
G,i
1
5.00 2.32e-5 .
.
.
.
1.91e-6
5.23e5 76
2
5.10 3.54e-5 .
.
.
.
2.00e-6
5.01e5 71
3
5.20 2.98e-5 .
.
.
.
.
4.87e5 67
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
i
v
i
c
i
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
1500 25.90 6.54e-7 .
.
.
.
.
.
.
This is what we need for a variety of analyses. First, it is useful to observe the
plot of M
i
versus elution volume: