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Outer peristaltic consept(10 within diameter of 300mm):

How the tubing should be for the pump if peristaltic is used. https://www.amazon.com/Stenner-EC30H-2-Peristaltic-Assembly-Ferrules/dp/B01AIUGLCW?ref_=fsclp_pl_dp_4

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With this type of connector(one counter clockwise, other clockwise:

Male Luer Lock to Barb Connector - 11563Image Added

To seal the internals of the flow chamber.