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d) Mass spectrometry combined with proteases
a) Specific enzyme A (commonly
trypsin
, which cleaves after K
(Lysine) and R (Arginine)
..ATFDE
K
LVVPAWGG
R
TTSIDPACVILFAA
K
LTAGGDPAE
R
V..
..ATFDEK LVVPAWGGR TTSIDCVILFAAK LTAGGDER V…
MALDI MS: Exact mass of fragments